Post-transcriptional gene silencing of chalcone synthase in transgenic petunias, cytosine methylation and epigenetic variation
نویسندگان
چکیده
منابع مشابه
Post-transcriptional silencing of chalcone synthase in petunia using a geminivirus-based episomal vector.
A vector that produces DNA replicons (multicopy plant episomes) was constructed using elements of the geminivirus tobacco yellow dwarf virus (TYDV). All plant cells contain an integrated chromosomal T-DNA copy of the TYDV elements that provides a template for the production of episomes in the cell nucleus. Transgenic Petunia hybrida plants containing a CaMV 35S promoter-driven chalcone synthase...
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Repeated DNA sequences are frequently mutated during the sexual cycle in Neurospora crassa by a process named repeat-induced point mutation (RIP). RIP is often associated with methylation of cytosine residues in and around the mutated sequences. Here we demonstrate that this methylation can silence a gene located in nearby, unique sequences. A large proportion of strains that had undergone RIP ...
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Epigenetic regulation of gene expression is a heritable change in gene expression that cannot be explained by changes in gene sequence. It can result in the repression or activation of gene expression and is therefore referred to as gene silencing or gene activation respectively. Until the end of the 1980s, only modifications of DNA or protein that lead to transcriptional repression or activati...
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genic bacterium notorious for its ability to redirect the plant metabolism to meet the pathogen’s gourmandise. This is achieved by the stable genetic modification of the host plant after integration of the bacterial T-DNA into the plant genome, a unique example of interkingdom DNA transfer. The T-DNA stems from the bacterial tumor-inducing (Ti) plasmid and travels as a single-stranded molecule ...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1999
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1999.00425.x